Effect of Growth Regulators In in vitro Propagation of Ixora spp.: An Short Review

Abstract

Various factors that affect culture establishment, shoot growth, proliferation, and rooting of Ixora were discussed in this article. A stem segment with a node from Ixora coccinea was best suited as an explant. Micropropogation of Ixora involves culturing shoot tips on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinin. The use of cytokinin alone had a low effect on the shoot morphogenesis of Ixora. The results showed that a lot of calli be induced in MS medium with 2,4-D (2.0 mg/l), increased shoots, and a little callus were induced in medium MS with BA (1.0 mg/l) and NAA (0.2 mg/l). It also indicated that axillary buds of Ixora treated with NAA and IBA, using any substrate as support, allowed reaching optimal morphology development, ensuring the survival and rooting of the plants in 100%. The use of NAA encourages the initiation of the root and the number and length of roots after 25 days of culture. The basal application of auxin to the cuttings of treated shoots improves rooting. By sequential reculturing and subculturing, 15-20 usable shoots (more than 1cm length) could be produced from the 2-node shoot segments after 12–15 weeks of culture. Microcuttings taken from In vitro proliferated shoots were rooted on a half-strength MS medium containing NAA, IBA, and IAA (0.1–0.5 mg/l). 90% of the plantlets can be established under the conditions when transferred to a specially made plastic tray containing coco-peat as a potting mix.