Microbial evaluation of cooked fufu sold in some major markets in Owo metropolis, Ondo state, Nigeria

Authors

  • Olalekan, Ajibolade Ogunoye Department of Pest Management Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria
  • Jacobs, Mobolade Adesina Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria
  • Maayo, Seth Ayodeji Department of Pest Management Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria.
  • Tomiwa, Ayobami Johnson Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria.
  • Ayodeji, Stephen Bolarinwa Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria
  • Oghenetega, Paul Okpewho Department of Botany, Delta State, Abraka, Delta State, Nigeria
  • Goodluck, Sunday Tomiye Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria

Keywords:

bacteria, fufu, yeast, microbes, microbial load

Abstract

Fufu (Cassava dough-like food) is one of Nigeria's most consumed staple foods, particularly in the southern parts of the country. This study aims to detect and quantify the microorganisms in cooked fufu sold in major Owo, Ondo State marketplaces. Freshly cooked fufu samples of about 50/gram wrapped in nylon were aseptically collected from sellers in three major Owo markets and transported to the Federal University of Technology, Akure (FUTA) Crop, Soil, and Pest Management Department lab for analysis. For bacteria, Nutrient Agar (NA) plates were incubated at 37+2ºC for 24 hours, whereas PDA plates for yeast and mould were incubated at 27+2ºC for 48 hours. Count and record the colonies generating spores (cfu/g) from viable bacterium cells and fungal spores per gram of each fufu sample after incubation. Purified cultures of each isolate were obtained through subcultures and stored at 4 ºC for biochemical testing. Cooked fufu samples found mostly lactic acid bacteria like Lactobacillus plantarum, L. fermentum, L. brevis, Leuconostoc mesenteroides, Streptococcus thermophiles, S. mutans, Pediococcus cerevisiae, Staphylococcus hominis, Bacillus pumilus, acidophilus, L. lactis, Micrococcus spp., B. subtilis, Diplococcus spp, S. epidermidis, and Bifidobacterium and two yeasts, Saccharomyces cerevisiae and Zygosaccharomyces spp. Some main markets in Owo metropolis sold cooked fufu samples with bacterial loads ranging from 0.2 to 2.3 × 107 CFU/g, and yeast loads from 1.6 to 4.55 × 107 CFU/g. Good hygiene can increase the safety and quality of cooked fufu sold at Owo main markets and lower the danger of food-borne illnesses.

Author Biographies

Olalekan, Ajibolade Ogunoye, Department of Pest Management Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria

OGUNOYE, Olalekan Ajibolade is an Assistant Lecturer in the Department of Pest Management Technology at Rufus Giwa Polytechnic, Owo, where he has been since 2020. On 11th April, 2023 he was appointed as member of the Faculty of Agricultural Technology Seminar Committee. From 2020 to 2022 he served as Department Project/Seminar Coordinator for ND/HND programmes at the defunct Crop, Soil and Pest Management Technology. During the maiden edition of 55th Annual Conference of Agricultural Society of Nigeria in 2021 he was appointed as Assistant Secretary/Member of Local Organizing Committee (LOC). He was equally a member of ASN Local Organizing Committee (LOC) Sub Committee on Technical and Paper Presentation. He received a B.Sc from University of Benin, Benin City in 2016, where he studied  Plant Biology and Biotechnology and graduated with Second Class Upper Honor. He got his M.Sc degree in Plant Pathology from the University of Ibadan, where he graduated with distinction in 2020. His research interests span across isolation and morphological identification of pathogens associated with vegetables; mycobiology and mycotoxins detection in Nigerian local foods; and molecular identification/diversity and identification of virulence-associated genes with pathogens isolated from vegetable crops. Much of his work has been on improving the understanding the microbial load, diversity and interaction between disease-causing agents and host pant in relations to the environment, management/control of plant diseases, specifically, it's influence in solving food shortages in the world today. He has attended many academic conferences/seminars. As of 2022, ResearchGate reports 4 publications, hundreds of reads and 1 citation to his work.

Jacobs, Mobolade Adesina, Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria

Adesina Jacobs Mobolade is a lecturer and Head of the Department of Crop Production Technology at Rufus Giwa Polytechnic, Owo, Nigeria.

Maayo, Seth Ayodeji, Department of Pest Management Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria.

Mr Ayodeji, Maayo Seth is an entomologist and Lecturer III in the Department of Pest Management Technology, Rufus Giwa Polytechnic Owo.

Tomiwa, Ayobami Johnson, Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria.

Mr Johnson, Tomiwa Ayobami, is an Assistant lecturer in the Department of Crop Production Technology at Rufus Giwa Polytechnic Owo. He is currently an exam officer for the department.

Ayodeji, Stephen Bolarinwa, Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria

Mr Boralinwa, Ayodeji Stephen, is a technologist in the Department of Crop Production Technology at Rufus Giwa Polytechnic in Owo, Ondo State, Nigeria. 

Oghenetega, Paul Okpewho, Department of Botany, Delta State, Abraka, Delta State, Nigeria

Mr. OKPEWHO, Oghenetega Paul is a lecturer in the Department of Botany, Faculty of Science, Delta State University, Abraka, Delta State, Nigeria. 

Goodluck, Sunday Tomiye, Department of Crop Production Technology, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria

Mr Tomiye, Goodluck Sunday is a student of the Department of Crop Production Technology at Rufus Giwa Polytechnic Owo.

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Published

2023-12-29

How to Cite

Ogunoye, O. A., Adesina, J. M. ., Ayodeji, M. S., Johnson, T. A., Bolarinwa, A. S. ., Okpewho, O. P., & Tomiye, G. S. . (2023). Microbial evaluation of cooked fufu sold in some major markets in Owo metropolis, Ondo state, Nigeria. Journal of Current Opinion in Crop Science, 4(4), 185–189. Retrieved from https://jcocs.com/index.php/ej/article/view/220

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Section

Research Article