Relative efficiency of chromosome elimination techniques for haploid induction parameters in triticale × wheat derived advanced generation through Zea mays- and Imperata cylindrica- mediated systems

Abstract

The study was undertaken to evaluate the relative efficiency of doubled haploid production methods following the chromosome elimination approach utilizing maize- and Imperata cylindrica- mediated systems. For doubled haploid production, triticale x wheat derived recombinants in different generations comprised of F₁, F₂, F₃, F₄, BC₁F₁, BC₁F₂, BC₁F₃, BC₁F₄ and BC₂F₃ were used. The haploid embryos were cultured in the MS medium with different mineral constituents and hormones. All the generations responded for different haploid induction parameters following maize- and Imperata cylindrica- mediated systems. Advanced generations responded better than early generations for haploid induction through both systems. All the haploid induction parameters, Imperata cylindrical- were performed significantly better than maize.